Figure 7.

Effect of the i.v. transplantation of DP296F treated with/without RTA402 (DP296F+RTA402 or DP296F) on the locomotor function of mice with a compression spinal cord injury (SCI). (A) The locomotor function of the hind limbs was evaluated weekly for 5 weeks after injury. The values are expressed as individual scores with mean lines. Significant differences from the control group (SCI mice with PBS injection) were determined using two-way repeated-measures ANOVA with post-hoc Tukey’s multiple comparison test. n = 8–10 for each group. (B) At 5 weeks after cell transplantation, an immunohistochemical analysis was performed using the central sagittal section of the spinal cord tissues. The lower panels depict a higher magnification of the boxed regions in the upper panels. Note that a measurable number of 5-HT⁺-nerve fibers were detected in the DP296F+RTA402 groups (three out of four), but not in the remaining groups (0 out of four) below the caudal edge of the injury sites. Single scale bar, 500 μm; double scale bars, 100 μm; respectively.