Figure 4.

Restoration of CD80/TNF-α double-positive cell recruitment on day 7 in the macrophage-depleted tooth extraction model. (A) Representative images of CD80 and TNF-α double-positive immunofluorescence staining in tooth extraction models as a reference for high-magnification images for all groups. (B) Representative images of high-magnification double-positive CD80 and TNF-α staining in the control and clodronate groups and quantification of mean positive cell numbers of CD80⁺ TNF-α⁺ double staining per selected ROI size in the total tooth socket area. High-magnification images are shown. The inset shows the location of the high-magnification image, and the dotted line-bound area shows the extracted tooth socket area. Upper panel, control group. Lower panel, clodronate group. Scale bar: 100 μm. Statistical analyses were performed using 2-way ANOVA and Tukey’s multiple comparison method. n = 4.