Prognostic significance of lymphovascular space invasion in early-stage low-grade endometrioid endometrial cancer: a fifteen-year retrospective Chinese cohort study

Abstract

Objective

In 2016, the ESMO-ESGO-ESTRO consensus included LVSI (Lymph-vascular space invasion, LVSI) status as a risk stratification factor for stage I endometrioid endometrial cancer (EEC) patients and as one of the indications for adjuvant therapy. Furthermore, LVSI is included in the new FIGO staging of endometrial cancer (EC) in 2023. However, the data contribution of the Chinese population in this regard is limited. The present study aimed to further comfirm the influence of LVSI on the prognosis of early-stage low-grade EEC in a fifteen-year retrospective Chinese cohort study.

Methods

This retrospective analysis cohort included 702 EEC patients who underwent TAH/BSO surgery, total abdominal hysterectomy, bilateral salpingooophorectomy in Peking University People’s Hospital from 2006 to 2020. Patients were stratified based on LVSI expression status as: LVSI negative group and LVSI positive group. Clinical outcome measures related to LVSI, assessed with a univariate and multivariate Cox proportional hazards regression model.

Results

702 EEC patients with stage I and grade 1–2 were analyzed. 58 patients (8.3%) were LVSI-positive and 14 patients (2.0%) was relapse. Recurrence rates in LVSI-negative and LVSI-positive were 1.6% and 6.9%, respectively. 5-year disease-free survival (DFS) rate in LVSI-negative and LVSI-positive were 98.4% and 93.1%, respectively. These rates for 5-year overall (OS) survival in LVSI-negative were 98.9% while it was 94.8% in LVSI-positive. Multivariate analysis showed that LVSI is an independent risk factor for 5-year DFS (HR = 4.60, p = 0.010). LVSI has a similar result for 5-year OS(HR = 4.39, p = 0.028).

Conclusions

LVSI is an independent predictor of relapse and poor prognosis in early-stage low-grade endometrioid endometrial cancer in the Chinese cohort.

Introduction

Endometrial cancer (EC) is the fourth most common gynecologic malignant tumor with over 66,200 estimated new cases at 2023 in the United States, while being the sixth most common gynecological cancer worldwide [1]. From 2000 to 2015, the incidence and mortality of carcinoma of corpus uteri have increased among Chinese women [2]. In general, most patients with endometrial cancer have good prognosis, excluding some special pathological types, such as serous endometrial carcinoma, clear cell carcinoma, etc. Endometrioid endometrial cancer (EEC) accounts for 80% of all endometrial cancers, and a large proportion of patients are at early-stage, low-grade more than 60% [3, 4]. However, the prognosis of early-stage, low-grade EC patients is still affected by a series of risk factors, but the research data are still few, and the relevant data of the Chinese population cohort are even less.

Lymph-vascular space invasion (LVSI) is described as the occurrence of cancer cells in the lymphatic and circulatory systems’ vessels. According to some studies, LVSI is an independent risk factor for lymph node metastasis (LNM) in EC and is considered a predictor of LNM [5–8]. Previous research has shown that LNM is predictive in preoperative assessment and prognosis [9–11]. In recent years, the prognostic value of LVSI in patients with endometrial cancer without lymph node metastasis or confined to the uterus has been gradually explored. Some studies report that LVSI significantly reduces overall survival (OS) and disease-free survival (DFS) in EC patients, particularly in early-stage EC [12–17]. In contrast, other studies suggest that LVSI does not significantly impact survival outcomes [18–22]. Among LNM-negative patients, clinical outcomes are similar regardless of LVSI presence [20].

According to the 2023 International Federation of Gynaecology and Obstetrics (FIGO) staging system, LVSI is included in the standards of stage I with or without focal LVSI and Stage II associated with substantial LVSI distribution [23]. The revision of 2023 FIGO staging confirmed the important role of LVSI in the prognosis of stage I and stage II patients [24, 25]. However, the impact of LVSI on the prognosis of l early-stage, low-grade endometrial cancer is still lack of more evidence, especially the cohort data of more than 10 years in Chinese population. This study aims to determine whether LVSI impacts the prognosis of patients with low-grade and early-stage endometrioid endometrial cancer, mainly gathered in women with stage I (IA and IB), Grade 1/2. Our analyses rely on clinical participant data who underwent surgery and their follow-up data to associate LVSI with EC prognosis.

Materials and methods

After obtaining authorization from the Ethics Committee of Peking University People’s Hospital (Institutional Review Board Approval Number: 2019PHB031-01, 8 March 2019), We analyzed their basic, hospital, and follow-up information. The population of this study included patients with endometrioid histology grade 1 or 2 and EC at stage IA or IB, as established by their final pathology reports. We first excluded patients with incomplete medical records (n = 63). We then excluded patients with tumors more advanced than stage IB after surgical staging (n = 201) and grade 3 diseases (n = 111). Women with other tumors were also excluded (n = 5) (Fig. 1).

Fig. 1.

Flowchart describing participant selection in the study. ^aEC: endometrial cancer

Gynecological oncologists performed total hysterectomy, bilateral salpingo-oophorectomy, selective bilateral pelvic and para-aortic lymphadenectomy, and pelvic washing on all eligible patients. We collected data on demographic, clinical, and surgical variables and evaluated them in this study. This included each patient’s age at first diagnosis, menopausal status, recurrence, time to recurrence, adjuvant treatment, follow-up duration, and survival time. We reviewed all original pathology reports and extracted pathological information. Experienced independent gynecological pathologists reviewed the pathology slides. We discussed controversial cases in expert meetings to reach a final decision. We assessed the grade, primary diameter of the tumor, and LVSI. All cases were staged according to the 2009 FIGO staging system [23]. Patients treated before 2009 were restaged based on clinicopathological data. Tumor histology and grade were assessed according to the World Health Organisation (WHO) classification system and FIGO criteria [26]. LVSI was diagnosed by identifying adenocarcinoma of any grade in endothelium-lined channels of uterine specimens at surgery [27]. We followed up with all patients after surgery through outpatient visits or phone calls. Telephone follow-up was conducted for patients regularly consulted at other hospitals. We collected information on symptoms and results from pelvic ultrasound, computed tomography (CT), or magnetic resonance imaging (MRI). We recorded any recurrences and deaths during the follow-up. If telephone follow-up revealed abnormal findings, patients were asked to undergo detailed examinations at our center. A diagnosis of relapse was made accordingly. Reasons for loss to follow-up included death from EC or other causes, loss to follow-up, and reaching the end of follow-up (October 31, 2022). Overall survival (OS) is the time from surgery to death from any cause, including a tumor. DFS was the time from surgery to confirmed relapse of EC. In survival analysis, patients without an event at their last follow-up were censored.

We compared clinicopathological factors between LVSI-negative and LVSI-positive patients. We used the student t-test and χ2 test for comparing continuous and categorical variables, respectively. Kaplan-Meier survival analyses (log-rank tests) and univariate Cox regressions were used to identify potential prognostic indicators. Multivariate Cox regression was used to analyze the survival influence of LVSI. In these regressions, we included factors with p < 0.05 in univariate analyses, in addition to LVSI. We examined the proportional hazard hypothesis with time-dependent covariates in all Cox regression models. Stepwise Cox regressions helped eliminate multicollinearity between variables, selecting important survival predictors for patients. Variables included were those with p < 0.05 in univariate Cox regressions. The analysis method was forward-biased: conditional, with an entry criterion for variables at p < 0.05. We conducted statistical analyses using SPSS software (version 27.0; IBM Corporation, USA), setting a significance level at p < 0.05.

Results

We enrolled a total of 702 eligible EC patients in this study. Of the 702 cases, the study population had a median age of 55 years (range 24–83) and the cases were followed up for a median of 62.4 months (range 0.4–195). Fifty-eight patients (8.3%) present LVSI positve. All patients underwent lymphadenectomy of both pelvic and para-aortic regions. After surgery, 235 patients (33.5%) received adjuvant therapy.

We displayed demographic and clinicopathological characteristics of low-grade and early-stage EEC women, based on LVSI status (Table 1). Patients testing positive for LVSI were more likely to have more extensive myometrial invasion (p < 0.001) and increased tumor size (p < 0.001) compared to those testing negative for LVSI. Compared to LVSI-negative patients, those who were LVSI-positive were more likely to receive adjuvant treatment (197/644 vs. 38/58, respectively, p < 0.001) and experience relapse during follow-up (10/644 vs. 4/58, respectively, p = 0.022).

Table 1.

Demographic and clinicopathological characteristics of low-grade and early stage endometrioid endometrial cancer patients (n = 702) according to LVSI status

Characteristics	Total	LVSI(-) n = 644	LVSI(+) n = 58	p value
Age(years, median)	55.23 ± 0.35	54.79 ± 0.36	60.10 ± 1.10	0.126
< 60	484(68.9)	452(70.2)	32(55.2)
≥ 60	218(31.1)	192(29.8)	26(44.8)
BMI				0.485
< 24	216(30.8)	197(30.6)	19(32.8)
≥ 24 and < 28	243(34.6)	220(34.2)	23(34.6)
≥ 28	243(34.6)	227(35.2)	16(27.6)
Menopausal status				0.013
Pre-menopausal	264(37.6)	251(39.0)	13(22.4)
Post-menopausal	438(62.4)	393(61.0)	45(77.6)
Grade				0.049
G1	341(48.6)	320(49.7)	21(36.2)
G2	361(51.4)	324(50.3)	37(63.8)
Depth of MI				< 0.001
≤ 50%	597(85.0)	563(87.4)	34(58.6)
> 50%	105(15.0)	81(12.6)	24(41.4)
PTD (cm), median				< 0.001
≤ 20 mm	363(51.7)	347(53.9)	16(27.6)
> 20 mm	339(48.3)	297(46.1)	42(72.4)
Adjuvant treatment				< 0.001
No	467(66.5)	447(69.4)	20(34.5)
Yes	235(33.5)	197(30.6)	38(65.5)
Relapse				0.022
No	688(98.0)	634(98.4)	54(93.1)
Yes	14(2.0)	10(1.6)	4(6.9)

LVSI, lymphovascular space invasion; MI, myometrial invasion; PTD, primary tumor diameter

LVSI was identified as a poor prognostic factor for the risk of relapse (Fig. 2). The study found that the 5-year disease-free survival rate was 98.4% for patients who tested negative for LVSI and 93.1% for those who tested positive (p = 0.005). The correlation of LVSI with OS in patients was also established (Fig. 3). The 5-year overall survival rate for LVSI-negative patients was 98.9%, compared to 94.8% for LVSI-positive patients (p = 0.017).

Fig. 2.

DFS of patients with negative and positive LVSI. ^aDFS: disease-free survival; LVSI: lymphovascular space invasion

Fig. 3.

OS of patients with negative and positive LVSI. ^aOS: overall survival; LVSI: lymphovascular space invasion

We found that LVSI and Grade are two prognostic factors for EEC patients’ DFS. According to univariable Cox regression analysis, the presence of LVSI was linked to poorer DFS (HR = 4.60; 95% CI = 1.44–14.66; p = 0.010), as was tumor grade (HR = 5.99; 95% CI = 1.25–25.02; p = 0.024). On multivariable analysis, the presence of LVSI was independently related to reduced DFS (HR = 3.82; 95% CI = 1.19–12.22; p = 0.024). Meanwhile, tumor grade was also identified as an independent risk factor for reduced DFS (HR = 5.04; 95% CI = 1.12–22.67; p = 0.035) (Table 2).

Table 2.

Univariate and multivariate analysis for diease-free survival in women with low grade and early stage endometrioid endometrial cancer

	Univariate			Multivariable
Characteristics	HR	p		HR	p
	(95%CI)			(95%CI)
Age
< 60	1
≥ 60	2.29(0.80,6.53)	0.121
Menopausal status
Pre-menopausal	1
Post-menopausal	3.63(0.81,16.24)	0.091
Grade
G1	1			1
G2	5.99(1.25,25.02)	0.024		5.04(1.12,22.67)	0.035
Depth of MI
< 50%	1
≥ 50%	1.59(0.44,5.70)	0.477
PTD (mm), median
≤ 20	1
> 20	1.14(0.96,1.36)	0.139
LVSI
No	1			1
Yes	4.60(1.44,14.66)	0.010		3.82(1.19,12.22)	0.024
Adjuvant
treatment
No	1
Yes	2.57(0.89,7.40)	0.081

DFS, diease free survival; HR, hazard ratio; CI, confidence interval; LVSI, lymphovascular space invasion; MI, myometrial invasion; PTD, primary tumor diameter

We also identified prognostic factors for OS (Table 3). In univariable Cox regression analysis, compared with other factors, LVSI positivity was more likely to reduce OS (HR = 4.39; 95% CI = 1.17–16.49; p = 0.028), as was age (HR = 3.32; 95%CI = 1.05–10.48; p = 0.040). On multivariable analysis, LVSI presence, compared to its absence, was independently associated with worse OS (HR = 3.87; 95% CI = 1.02–14.65; p = 0.046) (Table 3).

Table 3.

Univariate and multivariable Cox regression analysis of select covariates for OS

	Univariate			Multivariable
Characteristics	HR	p		HR	p
	(95%CI)			(95%CI)
Age
< 60	1			1
≥ 60	3.32(1.05,10.48)	0.040		3.08(0.97,9.78)	0.056
Menopausal status
Pre-menopausal	1
Post-menopausal	3.10(0.68,14.13)	0.145
Grading
G1	1
G2	0.94(0.30,2.90)	0.908
Depth of MI
< 50%	1
≥ 50%	0.52(0.07,4.00)	0.526
PTD (mm), median
≤ 20	1
> 20	1.13(0.95,1.35)	0.177
LVSI
No	1			1
Yes	4.39(1.17,16.49)	0.028		3.87(1.02,14.65)	0.046
Adjuvant
treatment
No	1
Yes	2.68(0.85,8.45)	0.093

OS: overall survival; HR, hazard ratio; CI, confidence interval; LVSI, lymphovascular space invasion; MI, myometrial invasion; PTD, primary tumor diameter

Discussion

This study analyzed the effect of LVSI on the prognosis of patients diagnosed with early-stage, low-grade EEC. In a cohort of 1083 EC patients, we focused on those with grade 1–2, stages IA and IB, and without other tumors. This criterion led to the selection of 702 EEC patients. After analyzing the statistics, we found (1) an LVSI prevalence of 8.3% among the 702 patients, (2) the presence of LVSI potentially impacting OS and DFS rates in early-stage and low-grade EEC patients.

The prognosis of EC is influenced by various factors, including LVSI, which is considered a significant prognostic indicator, especially in patients with early-stage and low-grade EEC [27]. The 2023 FIGO staging system acknowledges LVSI as a significant factor in assessing EC [28, 29]. Previous studies report LVSI incidences ranging from 8.9 to 13.5% in stage I EC patients [13, 18]. Our findings show that LVSI-positive patients have a lower DFS survival compared to the LVSI-negative group. Furthermore, LVSI positivity significantly affects OS in early-stage and low-grade EC patients. These findings is not conflict with our previous published cohort research, which concluded LVSI did not significantly impact EC prognosis, including all types and stages of endometrial cancer [18]. In some studies, LVSI positivity was classified into two categories: focal LVSI and diffuse LVSI. Focal LVSI indicates one or two vessels affected by the tumor found near it, while diffuse LVSI involves three or more vessels around the tumor or extensive LVSI in the myometrium with a spray-like growth pattern, irrespective of myometrial invasion severity [12]. This classification is valuable and precise, and widely utilized in clinical studies.

Except for LVSI, we identified tumor grade as another independent risk factor for the DFS rate in early stage EEC. Previous studies have not explored the correlation between the DFS rate and tumor grade in low-risk EEC [17]. After expanding the patient population, a correlation between tumor grade and DFS rate was identified. In high-grade EC, LVSI indicated statistically significant differences based on molecular classification, providing a more accurate prognosis [30]. Tumor grade is a crucial factor in early-stage and low-grade EEC prognosis.

The study found that 65.5% of LVSI-positive patients received adjuvant treatment, compared to 30.6% of LVSI-negative patients. However, adjuvant treatment showed no association with OS or DFS rates in our study. A retrospective analysis in 2023 suggested that adjuvant treatment improved the OS of LVSI-positive stage IIC EC patients, especially those with grade 3 EEC [31]. Yet, it did not influence the survival or recurrence rate in early-stage and low-grade EEC patients.

In addition, LVSI is one of the independent risk factors for lymph node metastasis in early stage endometrial cancer. Furthermore, the status of lymph nodes can affect the prognosis of patients with endometrial cancer. In recent years, the adoption of sentinel node mapping ensues a higher identification of patients with nodal disease compared with lymphadenectomy [32]. Retrospective studies have shown that the 5-year DFS rate and 5-year OS rate of patients receiving sentinel lymph node mapping are similar to those receiving lymph node dissection. Sentinel lymph node mapping has no negative impact on the 5-year prognosis of early-stage high-intermediate and high-risk endometrial cancer [33].

Further prospective evidence of the relation between sentinel node and the prognosis with endometrial cancer patients is still needed.

What’s more, our study has some limitations. Firstly, this study still needs large sample data from multiple hospital centers. Secondly, refining LVSI classification into categories such as focal and diffuse LVSI is needed. Thirdly, due to the limited number of cases with recurrence, the replase site was not stratified. Finally, the data of sentinel lymph node mapping was not included in this cohort, as this technology has been introduced to our hospital since 2015. In the future, we still need to further collect data for relevant research to confirm the objectivity and completeness of this conclusion.

Conclusions

Overall, our findings suggest that LVSI significantly predicts DFS and OS in early-stage and low-grade EC in a fifteen-year retrospective Chinese cohort study, emphasizing its role in assessing recurrence risk. For LVSI-positive patients, prompt and adequate adjuvant treatment can effectively prevent recurrence and enhance prognosis.

Author contributions

Bowen Sun wrote the main manuscript text and prepared Figs. 1, 2 and 3. Xiaobo Zhang made the pathological diagnosis of LVSI patients.Yangyang Dong, Xingchen Li, Xiao Yang and Lijun Zhao collected the data.Yuan Cheng and Jianliu Wang revised the manuscript. All authors reviewed the manuscript.

Funding

This work was supported by National Natural Science Foundation of China (Grant No. 82173119) and Peking University Clinical Medicine + X Youth Project (PKU2024LCXQ025).

Data availability

No datasets were generated or analysed during the current study.

Declarations

Ethics approval and consent to participate

This study was approved by Ethics Committee in Peking University People’s Hospital under the number 2019PHB031-01 and was in accordance with the ethical standards formulated in the Helsinki Declaration.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.