Fig. 2.

The DKK1 antibody enhanced the antitumor activity of paclitaxel and attenuated chemotherapy-induced peripheral neuropathy. A, B Effects of DKK1 antibody on the antitumor activity of paclitaxel in xenograft mice (A) and MMTV-PyMT mice (B). **p < 0.01, ***p < 0.001, one-way ANOVA, n = 6/8. C-E) Flow cytometry of CD3⁺ (C), CD8⁺ (D), and GZMB⁺CD8⁺ (E) CD45⁺ cells in xenograft mice treated with vehicle, PTX, PTX plus DKK1 antibody, or DKK1 antibody. *p < 0.05, **p < 0.01, one-way ANOVA, n = 4. F Effects of the DKK1 antibody on paclitaxel-induced mechanical allodynia (left) and thermal hyperalgesia (right) in xenograft mice. **p < 0.01, ***p < 0.001 compared with control group; ^##p < 0.01, ^###p < 0.001 compared with PTX group; two-way ANOVA, n = 8. G Transmission electron micrographs of the sciatic nerves and quantification of the G-ratio in mouse sciatic nerves from xenograft mice treated with vehicle, PTX, PTX plus DKK1 antibody, or DKK1 antibody. **p < 0.01, one-way ANOVA, n = 3. H Western blot of PGP9.5 in the hind paw intraplantar skin from xenograft mice treated with vehicle, PTX, PTX plus DKK1 antibody, or DKK1 antibody. ***p < 0.001, one-way ANOVA, n = 4