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. 2005 May 16;102(21):7589–7594. doi: 10.1073/pnas.0502935102

Fig. 4.

Fig. 4.

The activation state of αvβ3 influences tumor growth characteristics. (a and b) Comparison of anchorage-independent growth of LNCaP-C4-2 control (vector only), αvβ3 WT, and αvβ3 S752P cells in soft agar. (a) Note larger colonies formed by WT cells. (Scale bar, 50 μm.) (b) Shown are mean number of colonies per field ± SD of 10–12 random fields in 3 experiments. (c and d) Migration toward Vn depends on integrin αvβ3 functional state and VEGF. Migration of LNCaP-C4-2 αvβ3WT and S752P cells was assessed in the presence or absence of the anti-αvβ3 blocking antibody, LM609, as indicated (c), or in the presence of VEGF-neutralizing antibody or nonimmune IgG control (d). Migrated cells were counted in 10–12 random fields at ×200 magnification. Data shown represent means ± SD of three experiments.