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Double labeling of HSV antigens in a cornea cross section from an HSV-infected mouse injected with a Cy5-labeled human recombinant monoclonal antibody, LSCM (pseudocolored digital image). (A) Immunofluorescence for HSV gD and gB appeared to be mostly but not exclusively located in the subepithelial plexus. (B) Punctate Cy5 labeling colocalized with immunoreactivity for the HSV glycoproteins. (C) Superimposition of the images in panels A and B. Scale bar = 40 μm.
