FIG. 4.

Double labeling of neuritic antigens in cornea cross sections from an HSV-infected mouse injected with a Cy5-labeled human recombinant monoclonal antibody, as seen by LSCM (pseudocolored digital image). Immunoreactivity for the axonal markers neurofilament (A), Gap43 (D), and synaptophysin (G) and for the Schwann cell marker CNPase (J) is shown. Cy5 labeling (B, E, H, K) appears to colocalize with the neural markers (C, F, I, L), suggesting that the passively transferred human monoclonal antibody localizes to corneal nerve fibers. Scale bar = 40 μm.