Fig. 1 |. Latent cells uptake FAs secreted by reactive astrocytes.

a, Heatmap showing total FA profiles of neutral lipid content generated by GC–MS in HCC1954 Pa and Lat cells (n = 4, each group). b, IF image showing reactive astrocytes (GFAP⁺, magenta) surrounding Lat cells (GFP⁺, green) in mouse brain 5 weeks after intracardiac injection. c, IF image showing accumulation of LDs (red) in HCC1954 Pa and Lat cells (green) cultured with BODIPY-558/568-C12 labeled GFAP⁺ reactive astrocytes (gray). Scale bar, 100 μm. d, Quantification showing average LDs in Pa and Lat cells (HCC1954 and SKBR3) cultured with BODIPY-558/568-C12 labeled astrocytes for 24 h, n = 5 each group. e, Quantification showing time-dependent increase in lipid transfer from astrocytes to HCC1954 Pa and Lat cells, n = 6, each group. Isolated astrocytes from mice pups were labeled with C12-BODIPY(2 μM) for overnight and then washed three times. Next, HCC1954 Pa and Lat cells cultured in R3F were transferred to the astrocytes cultured plates and live cell imaging was performed using confocal microscope. d,e, Box and whiskers plot showing minima to maxima. with all points. f, TEM images showing LDs and mitochondria in HCC1954 Pa and Lat cells. g, Quantification of TEM image showing mitochondrial length in HCC1954 (Pa, n = 6; Lat, n = 6) and SKBR3 (Pa, n = 6; Lat, n = 5) model. h, Anti-TOMM20 antibody IF images showing mitochondrial morphology of HCC1954 Pa and Lat cells. Scale bar, 50 μm. i, Classification of cells based on percentage distribution of tubular, intermediate and punctate mitochondrial morphology in HCC1954 Lat and Pa cells. n = 8, each group. j, Western blot showing expression of DRP1 along with phosphorylated p-DRP1^S616(activated form) and p-DRP1^S637 (inactivated form) in HCC1954 Pa and Lat cells. In a, d, e, g and i, ‘n’ represents biologically independent samples. Data are presented in d, e, g and i as mean ± s.e.m., and P values in d, e and g were calculated by two-tailed unpaired t-test. The experiments shown in b, c, f, h and j were repeated independently at least two or three times with similar results.