Fig. 6 |. Genetic depletion or pharmacologic inhibition of DRP1 attenuates brain metastasis.

a, IF images showing mitochondrial morphology of HCC1954 Ctrl, DRP1-depleted and DRP1-rescued M-BM cells. b,c, Ex vivo brain images (b) and brain-only photon flux (c) showing metastatic burden in mice bearing Ctrl (n = 10), DRP1-depleted (n = 9) and DRP1-rescued (n = 9) M-BM cells. d, Quantification of GFP⁺ brain tropic latent metastatic cells/lesions in mice bearing HCC1954 Lat cells treated with vehicle (10% DMSO in corn oil) and Mdivi-1 (40 mg kg⁻¹) for 4 weeks (once daily). n = 4, each group. e,f, Ex vivo brain images (e) and brain-only photon flux (f) showing metastatic burden in mice bearing M-BM cells. After injection of M-BM cells, mice were treated with either vehicle (10% DMSO in corn oil, n = 8), or Mdivi-1 (40 mg kg⁻¹, n = 7) for 4 weeks (once daily). In c, d and f, ‘n’ represents number of mice, and data are presented as mean ± s.e.m. P values in c, d and f were calculated by Kruskal–Wallis test, two-tailed unpaired t-test and two-tailed Mann–Whitney U-test, respectively. The experiment shown in a was repeated independently two times with similar results.