FIG. 2.

Incorporation of the Vpr-IN fusion protein into IN-minus virions. (A) pSG3^S-IN and pSG3^wt were separately cotransfected into 293T cells with pLR2P-vprIN², pLR2P-vpr-IN, and pLR2P (vector alone), respectively. As described earlier (41), the extracellular progeny virions were concentrated from the supernatants of each culture by ultracentrifugation, lysed, and analyzed by immunoblot analysis using anti HIV-1 IN peptide antibody (A), human anti-HIV-2 antiserum (B), anti-Vpr (C), and anti-Gag (D) antibodies as probes.