Fig 8. Immunophenotyping reveals subtle changes in infected wild type mice compared to infected Pou2f3^-/- mice.

Male (●) and female (◼)Pou2f3^{+/+, +/-, -/-} mice were infected with 5 cysticercoids of H. diminuta and assessed at days post-infection (dpi). In (C, D), mice were treated with praziquantel (PZQ; 1 mg/mouse by oral gavage) at 8 dpi prior to necropsy. GATA3⁺ CD4⁺ lymphocytes and Foxp3⁺ CD4⁺ lymphocyte populations were analysed by flow cytometry using single cell suspensions isolated from the (A-D) mesenteric lymph nodes and (E-H) Peyer’s patches (without ConA stimulation) and presented as (A, C, E, G) a percentage of total live CD4⁺ lymphocytes and (B, D, F, H) as cell numbers. Data are mean ± SEM, n = 3-10/group, pooled from 1–3 experiments, * p<0.05 compared to genotype matched uninfected (Con) mice analysed by two-way ANOVA followed by Dunnett’s post-test and # p<0.05 analysed by multiple t tests with Hom-Sidak correction comparing between genotypes.