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. 2024 Jun 13;134(15):e165734. doi: 10.1172/JCI165734

Figure 6. A subset of LIN28’s RBP targets can rescue tumorigenesis in Lin28a/Lin28b/Tp53-TKO mice in part through increases in protein synthesis.

Figure 6

(A) Representative gross images of Lin28a/Lin28b/Tp53-TKO mice receiving NRASG12V in combination with pT3-RBPs (n > 3), pT3-eGFP (n = 5), or pT3-Luciferase (n = 5) for 7 weeks. Scale bars: 1 cm. All the images can also be found in Supplemental Figures 5B and 9. (B) Surface tumor number for A. One-way ANOVA was performed. (C) Liver-to-body weight ratios for A. One-way ANOVA was performed. (D) WB analysis quantified OP-puro labeling protein in Huh7 cells with Lin28b knockdown plus target overexpression. Number below the box shows relative intensity. (E) Representative gross images of Tp53-KO (Albumin-Cre; Tp53fl/fl; n = 5) and Lin28a/Lin28b/Tp53-TKO (Albumin-Cre; Tp53fl/fl; Lin28afl/fl; Lin28bfl/fl) mice that received NRASG12V by HDT for 7 weeks. TKO mice were subjected to BAZ2A inhibitors (n = 6) or DMSO (n = 5) once per week starting 3 days after HDT. Scale bars: 1 cm. One-way ANOVA was performed. (F) Schematic shows the importance of LIN28 proteins for tumor initiation. The image was designed and drawn using BioRender. **P < 0.01; ****P < 0.0001.