FIGURE 1.

Panx1 expression is higher in LECs of male mice. Orthogonal views of co‐immunofluorescent staining for LYVE‐1 (red) and Panx1 (green) on lymphatic capillaries in ears (a), intestinal villi (b) and LNs (c). Nuclei were stained with DAPI (blue). Asterisks indicate Panx1 and LYVE‐1 colocalization in the merged images. (d) Representative Western blot of Panx1 (41–48 kDa) in skeletal muscle of female and male mice. Red ponceau was used as a loading control. (e) As expected, Panx1 protein expression is higher in skeletal muscle of male mice (one‐tailed Student's t‐test; N = 3). (f) Representative image of the gating strategy used to obtain a pure population of LECs (red). (g) Panx1 mRNA expression in LECs extracted from LNs is equal in female (N = 4) and male (N = 3) mice. (h) Panx1 protein expression is higher in LECs of male mice (one‐tailed Student's t‐test; N = 4).