Figure 2:

A) Cryo-EM density map and atomic model of D10-NT fibrils. Three layers of the fibril structure are shown. B) Stick representation of a single layer of the D10-NT ordered fibril core (P42-G75). Disease linked mutations (red) S59L and G66V are situated in the interior of the protofilament fold, and V57E and G58R in the protofilament interface. In contrast, amino acids that differ between D10 and D2 (green) are exposed on the surface of D10-NT fibrils. C) Cryo-EM density map colored according to local resolution. D) Cartoon representation of secondary structure in the D10-NT fibril core. E) Sequence alignment of human D10 (top) and D2 (bottom) showing identical amino acids (middle), conservative substitutions (+) and non-conservative substitutions (blanks). The fibril core is boxed and shaded. Red lines indicate the C-termini of the N-terminal constructs used in our studies.