Figure 4.

FBLN3 cysteine mutations affect MMP2 zymography-based activities. (A) Stable ARPE-19 cells (lacking endogenous FBLN3, “FBLN3 KO”, same as those described in Fig. 2) were plated on 0.4 μm 12 well transwells followed by switching media to serum free conditions for 2 wks. Apical and basal aliquots of media were taken after 72 h of accumulation and analyzed on a 10% gelatin gel. (B) Quantification of MMP2 activity in different stable cell lines. n = 3 independent experiments, mean ± S.D. n.s. = not significant, * p < 0.05, ** p 0.01, *** p < 0.001, one-way ANOVA vs. FBLN3 KO cells with Dunnett’s multiple comparisons test. An additional one-way ANOVA was performed comparing 3xFT WT FBLN3 OE to G57C OE.