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Inefficiently secreted FBLN3 cysteine variants trigger increased endoplasmic reticulum (ER) stress. Stable ARPE-19 cells were analyzed by quantitative PCR (qPCR) for FBLN3, DNAJB9 (ERdj3), and HSPA5 (GRP78) transcript levels. Three to four independent data sets were combined for each panel, mean ± S.E.M, n.s. = not significant, * p < 0.05, ** p 0.01, *** p < 0.001, one sample t-test vs. hypothetical mean of 1 (i.e., unchanged).
