Fig. 2.

PCR amplification of H or L chain sequences from individual CD38+ cells. A representative set of 12 caps, each containing a microdissected CD38+ cell, was subjected to reverse transcription and nested PCR as described in Materials and Methods. The ≈600-bp H chain product (a) and ≈700-bp κ or λ L chain products (b) were visualized from most caps after electrophoresis in agarose gels. Size markers are indicated on the right.