Extended Data Fig. 6. Old male Il11−/−mice are protected from age-associated metabolic decline.
a Representative image of 108-week-old WT and Il11−/− male mice. b Body weights, c percentages of fat and lean mass (normalised to BW), d frailty scores, e body temperatures, f full body and forepaw grip strength measurements, g glucose and insulin tolerance tests (GTT and ITT) from young (12-week-old) and old (105-week-old) male WT and Il11−/− mice. h Respiratory exchange ratio (RER) measurement at day 2 (top; 24 h) and assessment of RER (second panel), cumulative food intake, and locomotive activities using the phenomaster system over a 5-day period in 68–70-week-old male WT and Il11−/− mice (n = 10/group). i Faecal caloric density as measured by bomb calorimetry in 95–105-week-old male WT and Il11−/− mice (n = 10/group). Indexed weight of j gastrocnemius k, soleus, l liver, m vWAT, subcutaneous WAT (scWAT), and BAT. b-g, i-m Data are shown as mean ± SD. b-g, j-m Two-way ANOVA with Sidak’s correction (young WT and Il11−/−, n = 6 (m (scWAT and BAT)), n = 9 (b-g, j-l, m (vWAT)); old WT, n = 12 (m (scWAT and BAT)), n = 15 (b-g, j-l, m (vWAT)); old Il11−/−, n = 12 (f-g, m (scWAT and BAT)), n = 14 (b-e,j-l, m (vWAT)); i two-tailed Mann Whitney test. AU: arbitrary units; BW: body weight; FC: fold change.