Extended Data Fig. 8. Therapeutic inhibition of IL11 reduces age-associated metabolic dysfunction, frailty and sarcopenia in female mice.
a Schematic of anti-IL11 (X203) therapeutic dosing experiment in old female mice for experiments shown in (b-h; IgG, n = 10 (g), n = 11 (b-f, h); X203, n = 13). Mice were given either X203 or an IgG control antibody (40 mg/kg, every 3 weeks) starting from 75 weeks of age for a duration of 25 weeks. Created with BioRender.com. b Body weights across time. c-d Changes (Δ; values at end-point (100-week-old) - values at starting point (75-week-old)) in c fat and lean mass percentage, and d area under the curve (AUC) of GTT and ITT. e Frailty scores at starting (75-week-old) and end-point (100-week-old). f-g Full body and front paw grip strength at end-point (100-week-old) and changes in full body and front paw grip strength over 25 weeks of treatment (values at end-point (100-week-old) - values at starting point (75-week-old)). h Body temperatures. b-d, f-h Data are shown as mean ± SD. b Two-way ANOVA, c-h two-tailed Student’s t-test except for d Δ AUC GTT, which was analysed by two-tailed Mann Whitney test. AU: arbitrary units; BW: body weight.