Extended Data Fig. 9. Beneficial effects of anti-IL11 in aged white adipose tissue.
a. Violin plot of Transcripts per million (TPM) values of senescence genes (based on Tabula Muris Senis consortium) in vWAT, liver, gastrocnemius samples from mice receiving either IgG or anti-IL11 as shown in schematic Fig. 3a. b. Relative Ucp1 mRNA from 10-week-old and 110-week-old male and female WT and Il11ra1−/− mice (vWAT). c. Heatmap showing row-wise scaled TPM values for the gene-list in Mitocarta 3.0. (no. of genes = 1,019 with TPM > = 5 in at least one condition). d. A lollipop plot for top 50 significant Mitocarta 3.0 pathways (p-adj<0.05) in enrichment analysis using fgsea R package. No negative NES was found to be significant. e. Distribution of RNA-seq reads at the Clstn3 locus from IgG or anti-IL11-treated vWAT. Relative Ucp1 mRNA expression levels in BAT from f therapeutic dosing group (75-week-old, n = 6; untreated 100-week-old, n = 6; IgG-treated 100-week-old n = 13; X203-treated 100-week-old, n = 12) and g female WT and Il11−/− mice (young WT, n = 5; young Il11−/−, n = 7; old WT and Il11−/−, n = 16/group). h Relative vWAT mRNA expression of pro-inflammatory markers (Ccl2, Ccl5, Tnfα, Il1β, Il6) in young (10-week-old) and old (110-week-old) male and female WT and Il11ra1−/− mice. a, c, d, e Liver and gastrocnemius (n = 8/group), vWAT IgG, n = 7; vWAT anti-IL11, n = 6; b, h young male WT, n = 8; young male Il11ra1−/−, n = 7; old male WT, n = 11; old male Il11ra1−/−, n = 14; young female WT, n = 7; young female Il11ra1−/−, n = 8; old female WT, n = 15; old female Il11ra1−/−, n = 12. a Data are shown as violin plots with median ± min-max; b, f-h data are shown as mean ± SD. b, g, h Two-way ANOVA with Sidak’s correction; e one-way ANOVA with Tukey’s correction.