Fig. 2. Female Il11-deleted mice are protected from age-associated obesity, frailty, and metabolic decline.
a, Representative photograph of 105-week-old female wild-type and Il11−/− mice. b–g, Body weight (b), percentage of fat and lean mass (normalized to body weight), frailty score (d), full body grip strength (e), serum cholesterol and triglycerides (f), and GTT and ITT (g) of young (12-week-old) and old (105-week-old) female wild-type and Il11−/− mice. h–j, Indexed vWAT and scWAT weight (h), relative vWAT mRNA expression level of Acc1, Fasn and Srebp1c (i), and western blot showing activation status of ERK1/2, p90RSK, LKB1, AMPK, mTOR, p70S6K and S6RP and protein expression levels of p16, p21 and GAPDH (j). n = 6 per group. Western blots for the respective total proteins are shown in Extended Data Fig. 5k. k,l, Telomere length and mtDNA copy number (l) in vWAT from young and old female wild-type and Il11−/− mice. b–i,k–l, Data are mean ± s.d. (young wild-type, n = 8; young Il11−/−, n = 9; old wild-type, n = 16; old Il11−/−, n = 18; except for h (scWAT): young wild-type, n = 5; young Il11−/−, n = 7; old wild-type and Il11−/−, n = 16). Two-way ANOVA with Sidak’s correction (b–f,h,i,k,l); two-way repeated measures ANOVA with Sidak’s correction (h). For gel source data, see Supplementary Fig. 1.