Extended Data Fig. 1. Age-dependent expression of IL11 in varied cell types across tissues.
a Western blots (WB) of total ERK1/2, p90RSK, LKB1, AMPK, mTOR, p70S6K, and S6RP in livers from 12, 25, 50, 75, and 110-week-old male mice for the respective phosphoproteins shown in Fig. 1b. b WB of IL11 and GAPDH in visceral gonadal white adipose tissue (vWAT) and gastrocnemius from 12, 25, 50, 75, and 110-week-old male mice (n = 5/group). c WB of p-ERK1/2, p-p90RSK, p-LKB1, p-AMPK, p-mTOR, p-p70S6K, p-S6RP, and their respective total proteins in gastrocnemius from 12, 25, 50, 75, and 110-week-old male mice (n = 5/group). d WB of IL11 and GAPDH in the liver, vWAT and gastrocnemius from 12-week-old and 110-week-old male and female mice (n = 3/group). e-g Representative immunofluorescence images (scale bars, 100 µm) of EGFP expression in the livers, vWAT, and gastrocnemius, colocalized with parenchymal cell markers Adiponectin (AdipoQ) in vWAT and Four and a half LIM domains (FHL1) in gastrocnemius, endothelial cells (CD31), smooth muscle transgelin (SM22α), and pan-fibroblast marker (PDGFRα) of 10 and 110-week old Il11-EGFP mice (representative dataset from n = 3/group).