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. 2024 Jul 18;15:1434573. doi: 10.3389/fphar.2024.1434573

TABLE 7.

Analysis of the putative metabolites of butonitazene in human liver microsomes and liver S9 fractions.

Ion/Fragment % relative intensity (sum intensity) % relative abundance (peak area) % relative intensity (sum intensity) % relative abundance (peak area)
Human liver microsomes Human liver S9
Butonitazene
Hydroxylation 0.4 0.4 0.7 0.7
N-Desethylation 51 50 28 27
Dealkylation 2.5 2.6 1.2 1.4
Desethylation + Dealkylation 16.3 16.3 8.1 8.6
Desethylation + Hydroxylation 1.7 1.9 1.3 1.6
Isotonitazene
Hydroxylation 0.5 0.5 0.3 0.3
N-Desethylation 27 33 30 25
Dealkylation 0.02 0.02 - -
N-Desethylation + Dealkylation 0.98 1.20 0.6 0.9
N-Desethylation + Hydroxylation 0.2 0.2 0.1 0.2
Depropylated-demethylated 1.2 1.4 0.9 0.8
N-De-ethylamination and Dealkylation 0.1 0.1 0.1 0.1
N-desethylation, debutylation 0.7 0.8 0.5 0.5
Protonitazene
Hydroxylation 0.3 0.4 0.5 0.4
N-Desethylation 41 37 31 30
Dealkylation 0.16 0.19 0.6 0.8
N-Desethylated + Dealkylated 0.71 1.05 0.7 0.9
Demethylated + Dealkylated 1.8 1.9 1.0 1.1
De-aminopentylated 0.08 0.09 0.4 0.5
N-Desethylation + Hydroxylation 1.0 1.0 1.0 0.9