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The addition of the NO donor SNAP inhibits the release of infectious virus into the culture supernatant. MDCK cells were infected with influenza virus A/Netherlands/202/95 at the indicated MOI and subsequently treated with 400 μM SNAP or NAP or left untreated. The measurement of infectious virus in culture supernatants of the cells was performed by limiting dilution analysis and expressed as 50% tissue culture infective doses per milliliter (TCID50/ml) as previously described (30).
