Fig. 6.
Restricted feeding ameliorates the IBD-like symptoms in mice by targeting the intestinal clock. Colon clock genes expression A and survival B in control, Bmal1IEC−/−, IL-10−/−BL6 and Bmal1IEC−/−xIL-10−/−BL6 mice under AD and RF conditions. Disease scores C, organ weights D, and representative H&E staining images of colon sections E from IL-10−/−BL6 and Bmal1IEC−/−xIL-10−/−BL6 mice under AD conditions. F Schematic illustration of the experimental RF design. Survival analysis G, representative H&E staining of colon sections H, histopathological scores I, immune cell frequency in the colonic LP J and Tnf expression K in IL-10−/−BL6 and Bmal1IEC−/−xIL-10−/−BL6 mice under AD and RF conditions. The data points represent individual mice (n > 6/time points for each group). One-way ANOVA, two-way ANOVA following Benjamini‒Hochberg correction, and three-way ANOVA followed by Benjamini–Krieger-–Yekutieli correction were used. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The data are presented as the mean ± SEM. Details on the number of mice per time point are summarized in Supplementary Table 6