Fig. 4.

Gingipain derived from P. gingivalis may degrade INSR in vitro. a Western blot analysis of changes in INSR protein expression in hepatocytes, myocytes, and adipocytes after coculture with 100 MOI P. gingivalis and semiquantitative analysis (the expression intensity of proteins in the control group was set as 1). b Immunofluorescence staining was used to detect changes in INSR protein expression (red) in hepatocytes, myocytes, and adipocytes after coculture with P. gingivalis and to determine the distribution of gingipain (green, indicated by white arrows) (scale bar: 50 µm). INSR, insulin receptor; *P < 0.05; **P < 0.01