Fig. 6.

Bag6-mediated regulation of Il33 protein level and release. A, B ELISA to detect IL33 in cell lysate or supernatant (SN) of WT/KO Pan02 cells pretreated with or without Kifunensine (KIF) (150 µM) in (A) or with GW4869 (20 µM) in (B). Treatment was performed for 24 h followed by replacement of medium and 24 h incubation (n = 3 ± SEM). C ELISA to detect IL33 in cell lysate and SN after rescue of the Bag6 expression in Bag6 KO Pan02 cells (n = 3 ± SEM). Statistical significance: (A–C) unpaired t-test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001