Fig. 7.

The secretome of MCs activated with KO-EVs promoted tumor growth. A The top 15 upregulated proteins in the secretome of human MCs pre-treated with PANC-1 KO-EVs (n = 2) are depicted. Normalized protein expression values (NPX) were averaged and the effect size calculated as compared to a PBS control is indicated (full protein list in Supplementary Table S1 and Fig. S6D for the global distribution of the effect size for the 15 upregulated proteins). B Kaplan Meier analysis of the top 15 upregulated proteins correlated with survival (TCGA data via GEPIA2). C Representative images of KPC mouse and PDAC human organoids after treatment with MC secretome from mouse/human MCs that were pre-treated with WT- or KO-EVs from Pan02 or PANC1, respectively as well αIL33 and αPDGF as indicated. D Quantification of organoid sizes determined via cell titer (mean ± SEM, n = 4–6). E The organoids were stained with anti-Ki67 antibodies (red), with Hoechst 33342 (nuclei, blue) and Alexa 546 phalloidin (actin, green) to visualize cellular structures. Scale bars: 10 µm. Statistical significance: (D) unpaired Mann–Whitney U test; *P < 0.05; **P < 0.01; ***P < 0.001