Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Step-by-step workflow for preparing negative stain grids of the purified C. crescentus flagella as detailed in the negative stain section. Numbering in the figure corresponds to numbering in Section C. All blotting steps are conducted as in step C5, by touching the edge of the grid to the filter paper. The procedure is repeated for replicate grids or different sample concentrations to be imaged by electron microscopy.
