Reagent	Final concentration	Amount
Peptone	0.2% (wt/v)	2 g
Yeast extract	0.1% (wt/v)	1 g
1 M MgSO4	1 mM	1 mL
1 M CaCl2	0.5 mM	0.5 mL
H2O	n/a	Fill up to 1 L
Total	n/a	1 L