Distribution coefficients of different dyes and net charge of major microspecies at pH 7.4.

Dye	DPBS	DMEM	MiliQ	Tris	Charge (pH: 7.4)
Rhod 6 G	5.65	5.65	5.64	5.65	0
NR	4.62	4.62	4.62	4.62	0
Rhod101	4.18	4.07	−2.85	−0.13	0
RhodB	3.01	2.89	−4.29	−1.39	0
EMA	2.71	2.61	0.97	0.39	−1
Cy7	2.45	2.34	−1.68	1.22	−1
Res	0.63	0.63	0.63	0.63	0
AZ	−0.31	−0.31	−0.29	−0.31	−1
Sulford	−0.85	−0.96	−4.98	−2.08	−1
Fl	−1.34	−1.34	−1.07	−2.00	−2
Cy5	−2.27	−2.38	−6.4	−3.5	−2
DAPI	−3.24	−3.46	−7.93	−5.5	+2
AF350	−5.92	−5.92	−5.66	−6.16	−2
Pyr	−6.33	−6.33	−6.31	−6.34	−3
AF488	−7.13	−7.13	−7.03	−7.53	−3
AF568	−7.20	−7.31	−13.64	−11.6	−3