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. 2024 Jul 1;86:101983. doi: 10.1016/j.molmet.2024.101983

Figure 3.

Figure 3

miPEP deficient mice are protected against diet induced obesity and glucose intolerance. (A) Experimental workflow HFHSD = High fat, high sugar diet. OGTT = Oral glucose tolerance test, LTT = Lipid Tolerance Test. Changes in (B) body weight, (C) fat mass and (D) adiposity pre-diet, at 2.5 wks and 8 wks of diet is shown. Mean ± S.D., N = 9–11, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (E) Representative dual energy X-ray absorptiometry (DEXA) image scan for control (miPEPfl/fl, fl/fl) and adipo-miPEP-KO (aKO) after 8 wks of diet intervention. (F) Food intake Mean ± S.D., p-value is reported. (G) Fasting triglycerides and (H) plasma NEFAs. Mean ± S.D., N = 5–6. ∗p < 0.05 vs miPEPfl/fl mice. (I) Kinetic and (J) area of the curve of lipid tolerance test using oral gavage of intralipids. Mean ± S.D., N = 5–6 (K) Triglycerides levels across tissues in miPEPfl/fl (fl/fl, grey) and adipo-miPEP-KO (aKO, light blue) mice. Mean ± S.D., N = 3–6. (L) Oral Glucose tolerance test, (M) Fasting glucose was determined after 8 wks of HFHSD. (N) Area of the curve was determined from the Oral Glucose tolerance test. (O) Fasting Blood insulin and at 15 min during the Oral Glucose tolerance test. (P) HOMA-IR (Q) Matsuda Index. Mean ± S.D., N = 8–10, ∗p < 0.05, ∗∗p < 0.01 vs miPEPfl/fl mice.