Deletion of miPEP increases muscle metabolism. (A) Experimental summary of the Dual Tracer Test (DTT) workflow and hypothetical data. (B) Blood glucose levels during the DTT. (C) Area of the curve after insulin administration in control (miPEPfl/fl, fl/fl) and adipocyte specific miPEP KO (aKO, light blue) mice. (D–H) Accumulation of basal [14C]2DG-6P and insulin-stimulated [3H]2DG-6P in Gonadal white adipose tissue (gWAT) (D), Subcutaneous adipose tissue (SAT) (E), Brown adipose tissue (BAT) (F), Heart (G), and skeletal muscle (H) was determined across genotypes. N = 5–6, ∗p < 0.05, ∗∗p < 0.01 vs miPEPfl/fl mice. (I) Experimental workflow for global proteomics of soleus muscle in miPEPfl/fl and aKO mice. (J) Abundance of Fbp2 in soleus, N = 5–6 Mean ± S.D. ∗∗p < 0.05 vs miPEPfl/fl.