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rIL-13–treated normal gastroids displayed both chief cell and SPEM cell markers. (A) Immunofluorescence staining for MIST1 (chief cell), PDX1 (an antral lineage marker), and CD44v9 (SPEM cell) in normal gastroids and Meta1 gastroids. Scale bar: 100 μm. (B) Quantitation of average percentage of MIST1-positive cells and PDX1-positive cells. Mean ± SD (N = 6–7 from 3 independent experiments). ∗∗P < .01, ∗∗∗P < .001.
