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. 2024 May 29;18(3):101366. doi: 10.1016/j.jcmgh.2024.101366

Figure 2.

Figure 2

Meta1 and Meta4 mouse gastroids displayed different behavior. (A) Top, representative bright field images of Matrigel domes containing Meta1 and Meta4 gastroids at 1 or 2 weeks after plating. Scale bar: 500 μm. Bottom, H&E staining shows cellular distribution in the Meta1 and Meta4 gastroids. Enlarged images show cellular organization (mono- or multilayering). Scale bar: 100 μm. (B) Quantitation of the average percentage of gastroids presenting budding formation in Meta1 and Meta4 gastroids at 1 or 2 weeks of in vitro culture. Mean ± SD (N= 8–10 from 3 independent experiments). ∗∗∗∗P < .0001. (C) Immunofluorescence staining of Meta1 and Meta4 gastroids after 2 weeks of in vitro 3D culture for SPEM cell markers (CD44v9 and AQP5) and a dysplastic cell marker (TROP2). Scale bar: 100 μm. (D) Quantitation of intensity units for different SPEM and dysplastic cell markers in gastroids on C. Mean ± SD (n = 4). ∗∗∗∗P < .0001.