Figure 8.

IL-13 maintained the mature SPEM phenotype in Meta1 gastroid cells in long-term ALI culture. (A) Schematic illustration of ALI culture procedure. (B) Representative bright field images of top view of ALI filters. Secretion and accumulation of mucins increase after 14 days of culture with rIL-13. At day 28, there was a tenacious mucus layer on top of the Meta1 gastroid cells that were treated with rIL-13. Scale bar: 200 μm. (C) Whole mount immunofluorescence staining for SPEM-related markers, GSII and CD44v9, showed significant increase of GSII coverage proportion in the rIL-13 treatment. Scale bar: 100 μm. Quantitation of GSII staining per cell area is shown on the right. Mean ± SD (N = 3 independent experiments). ∗∗P < .01.