Table 3.

Commercially available automated and manual extraction kits for the isolation of total RNA including small RNAs from low-input cell and tissue samples

RNA extraction kit	Manufacturer	Input	Method	RNA biotypes
QIAzol TRI Reagent TRIzol	QIAGEN Sigma-Aldrich Thermo Fisher Scientific	up to 100 mg per 1 mL reagent or 1 mL per 107 cells	manual	total RNA including miRNAs
RNeasy Plus Micro and Mini Kits	QIAGEN	5 × 105 cells or 5 mg (Micro kit) 107 cells or 30 mg tissue (mini kit)	manual and automated	total RNA including miRNAs
RNAqueous Total RNA and Micro Isolation Kit	Thermo Fisher Scientific	1–75 mg of tissue or from 102–107 cells	manual	total RNA including miRNAs
Arcturus PicoPure RNA Isolation Kit	Thermo Fisher Scientific	laser-capture micro-dissected samples and larger samples	manual	total RNA
MagMax mirVana Kit	Thermo Fisher Scientific	various low-input samples	manual and automated	total RNA including miRNAs
Total RNA Purification Plus Kit	Norgen	various low-input samples	manual	total RNA including miRNAs