Figure 5.

Cerulein-induced intrapancreatic protease activation in Spink1-KO^hetmice. C57BL/6N and Spink1-KO^het mice were administered a single injection (left panels) or 2 × 8 hourly injections (right panels) of cerulein. Control animals were given saline injections. Mice were euthanized 30 minutes after the single/last injection, the pancreas was homogenized, and protease activities were measured as described in Methods. (A) Intrapancreatic trypsin activity. (B) Intrapancreatic chymotrypsin activity. Individual values with the mean and standard deviation were graphed. The difference of means was analyzed by 1-way analysis of variance with Tukey’s post-hoc test.