Figure 1.

Aberrant protein changes and alternative splicing in men with poor semen quality

Human spermatozoa were collected from both fertile and infertile men. After lysis, the samples were digested and run through quantitative mass spectrometry.

(A) Principal component analysis showing separation of both fertile (green) and infertile (red) men based on the protein changes in the proteomic analysis.

(B) Extracted ion chromatogram of peptide derived from CAMP from 2 fertile (top) and 2 infertile (bottom) samples (C–E) sperm lysates from fertile and infertile men were lysed and run into SDS page. The samples were then probed for using the (C) anti-CAMP, (D) anti-HSP4AL, and (E) anti-MENT antibody. The membrane was then stripped and re-probed with anti-beta tubulin as a loading control. The position of the molecular mass markers are shown on the left hand side.