Figure 4.

Effects on tau aggregation and fibril binding of individual EVOO phenolic compounds. (a) ThT analysis of tau (20 μM) aggregation kinetics, alone and in the presence of equimolar concentrations of individual polyphenols (chemical structures shown in Figure 3). Means are shown of n = 3 measurements per group. (b) HPLC binding analysis of a defined mixture of standard EVOO phenolic compounds in the presence of tau filaments. Chromatograms are shown for a solution of 20 μM compounds alone (black) and after addition of 20 μM tau followed by sedimentation (blue). (c) HPLC binding analysis of EVOO phenolic compounds in the presence of Aβ40 fibrils. Chromatograms are shown for two solutions of 20 μM standard compounds alone (black) and after addition and removal by the sedimentation of 20 μM Aβ40 (red). Arrows highlight the extent of peak reduction after sedimentation.