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. 2024 Jul 17;9(30):32557–32578. doi: 10.1021/acsomega.4c01281

Table 5. Summary of the HPLC Analysis of EVOO Compound Binding (20 μg/mL Greek Extract) to Insoluble Aβ40 Fibrils (20 μM Monomer Equivalent).

retention time (min) compound normalized peak intensitya
% bound λ (nm)b
    –fibril +fibril    
1.0 hydroxytyrosol 12.9 3.8 71 275
1.2 unknown 11.7 1.7 86 275
1.7 unknown 1.3 0.0 100 275
1.9 tyrosol 93.1 8.4 91 275
2.9 vanillic acid 2.2 0.0 100 275
3.0 caffeic acid 1.6 0.0 100 275
5.7 p-coumaric acid 3.8 0.0 100 275
8.1 unknown 7.2 6.3 12 240
8.9 unknown 1.4 0.0 100 340
9.2 unknown 100.0 14.4 86 240
9.4 unknown 96.5 6.3 93 240
12.1 unknown 6.5 5.5 16 240
14.1 luteolin 3.6 1.2 66 340
14.3 unknown 11.8 7.2 39 275
14.6 (+)-pinoresinol 12.9 8.9 31 275
15.0 unknown 2.1 0.0 100 340
15.3 naringenin 70.3 55.5 21 275
15.8 unknown 2.4 0.0 100 275
16.0 apigenin 3.1 0.0 100 340
17.7 unknown 1.5 0.0 100 275
17.8 unknown 1.6 1.2 24 275
18.3 unknown 2.5 2.0 25 275
19.7 unknown 3.2 0.0 100 275
19.9 unknown 1.1 0.0 100 275
20.4 unknown 1.4 0.0 100 275
22.6 unknown 20.2 14.6 28 240
a

Normalized to the maximum peak intensity (at 9.2 min) in the absence of fibrils.

b

Wavelength of maximum absorbance chosen for quantification.