Table 5. Summary of the HPLC Analysis of EVOO Compound Binding (20 μg/mL Greek Extract) to Insoluble Aβ40 Fibrils (20 μM Monomer Equivalent).
retention time (min) | compound | normalized
peak intensitya |
% bound | λ (nm)b | |
---|---|---|---|---|---|
–fibril | +fibril | ||||
1.0 | hydroxytyrosol | 12.9 | 3.8 | 71 | 275 |
1.2 | unknown | 11.7 | 1.7 | 86 | 275 |
1.7 | unknown | 1.3 | 0.0 | 100 | 275 |
1.9 | tyrosol | 93.1 | 8.4 | 91 | 275 |
2.9 | vanillic acid | 2.2 | 0.0 | 100 | 275 |
3.0 | caffeic acid | 1.6 | 0.0 | 100 | 275 |
5.7 | p-coumaric acid | 3.8 | 0.0 | 100 | 275 |
8.1 | unknown | 7.2 | 6.3 | 12 | 240 |
8.9 | unknown | 1.4 | 0.0 | 100 | 340 |
9.2 | unknown | 100.0 | 14.4 | 86 | 240 |
9.4 | unknown | 96.5 | 6.3 | 93 | 240 |
12.1 | unknown | 6.5 | 5.5 | 16 | 240 |
14.1 | luteolin | 3.6 | 1.2 | 66 | 340 |
14.3 | unknown | 11.8 | 7.2 | 39 | 275 |
14.6 | (+)-pinoresinol | 12.9 | 8.9 | 31 | 275 |
15.0 | unknown | 2.1 | 0.0 | 100 | 340 |
15.3 | naringenin | 70.3 | 55.5 | 21 | 275 |
15.8 | unknown | 2.4 | 0.0 | 100 | 275 |
16.0 | apigenin | 3.1 | 0.0 | 100 | 340 |
17.7 | unknown | 1.5 | 0.0 | 100 | 275 |
17.8 | unknown | 1.6 | 1.2 | 24 | 275 |
18.3 | unknown | 2.5 | 2.0 | 25 | 275 |
19.7 | unknown | 3.2 | 0.0 | 100 | 275 |
19.9 | unknown | 1.1 | 0.0 | 100 | 275 |
20.4 | unknown | 1.4 | 0.0 | 100 | 275 |
22.6 | unknown | 20.2 | 14.6 | 28 | 240 |
Normalized to the maximum peak intensity (at 9.2 min) in the absence of fibrils.
Wavelength of maximum absorbance chosen for quantification.