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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: J Med Chem. 2019 Jun 25;62(13):6175–6189. doi: 10.1021/acs.jmedchem.9b00428

Figure 4.

Figure 4.

A) Effect of ezetimibe and pranlukast on the production of hrGALNS in E. coli. Enzyme activity was measured in the soluble fraction of the cell lysate after 24 a h induction. No effect of ezetimibe and pranlukast on GALNS activity was observed in the extracellular fraction. Results are reported as relative to those observed in the control (DMSO alone) treated cultures. B) Effect of ezetimibe and pranlukast (0.001 μM) on the production of hrGALNS in the yeast Pichia pastoris. GALNS activity was measured in the extracellular fractions at 24, 48, 72, and 96 h. No effect on GALNS activity was observed at higher concentrations of ezetimibe and pranlukast. The DMSO treated cultures were used as the control groups (n = 3, ANOVA Sidak t test ** p < 0.01, *** p < 0.001, **** p < 0.0001).