Fig. 2.

A CLSM images of APP^Gd-Cy5 (10 μM) in 4T1 cells treated with either 0 or 50 μM Ac₄ManNAz (Non-click-4T1 or Click-4T1), and HC11 cells treated with 50 μM Ac₄ManNAz (Click-HC11). B Relative FL intensities of APP^Gd-Cy5 (10 μM) in 4T1 cells treated with either 0 or 50 μM Ac₄ManNAz, and HC11 cells treated with 50 μM Ac₄ManNAz. (C) Relative FL intensities and (D) fluorescence images of DOX in 4T1 cells incubated with DOX, APP^Gd-DOX, and Click-APP^Gd-DOX (2 μM). DOX. E CLSM images of 4T1 cells treated with 10 μM APP^Gd-Cy5, co-stained with 5 μM DiO, or 100 nM Mito-Tracker or 50 nM Lyso-Tracker green. F Co-localization curves. Scale bars: 10 µm. Cell viabilities of (G) HC11 and 4T1 cells treated with Ac₄ManNAz, (H) HC11 cells or (I) 4T1 cells treated with DOX, APP^Gd-DOX, and Click-APP^Gd-DOX