Scheme 1.

Illustration of the bio-orthogonal click chemistry strategy for PD-L1-targeted imaging and pyroptosis-mediated chemo-immunotherapy of TNBC. A Chemical structure of PD-L1-targeted probe APP^Gd-Cy7 and prodrug APP^Gd-DOX. B In vivo MR/NIRF imaging process involves metabolism glycoengineering, and the PD-L1 targeting accumulation of APP^Gd-Cy7 in tumor tissue; In vivo synergistic chemo-immunotherapy strategy includes metabolism glycoengineering, PD-L1 targeting, and pH-responsive drug release and pyroptosis-mediated regulation of antitumor immunity