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. 2024 Aug 1;24(4):897–911. doi: 10.17305/bb.2024.9979

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© 2024 Pang et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 5. — Overexpressed JAM3 promoted BC cell migration and invasion abilities by regulating expression levels of EMT-related genes. (A and B) The fundamental expression of JAM3 in normal bladder cells and BC cells; (C and D) Wound heling assay for overexpressed T24 cells (C) and UMUC3 cells (D); (E and F) Statistical results of wound healing assay for T24 cells (E) and UMUC3 cells (F); (G and H) Migration and invasion assay for overexpressed T24 cells (G) and UMUC3 cells (H); (I and J) Overexpressed JAM3 upregulated levels of N-Cadherin and MMP-2 proteins, while inhibited the level of Claudin-1 protein in T24 cells (I) and UMUC3 cells (J); (K and L) The relative protein expression level of EMT-related proteins, calculation followed protocol as below: gray levels of targeted proteins divided by gray levels of paired tubulin protein. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NC: Negative control group; OE: Overexpressed group; JAM3: Junctional adhesion molecule 3; UMUC3: Bladder cancer cell line; EMT: Epithelial–mesenchymal transition; BC: Bladder cancer.