Figure 6.

Sirt6 overexpression attenuated HG induced oxidative stress, mitochondrial dysfunction, ferroptosis and podocyte injury. (A–F) Podocytes were transfected with pcDNA3.1, pcDNA3.1 Sirt6, Scrambled siRNA, Sirt6 siRNA and then stimulated with HG (30 mM) for 48h. Western blots and quantitative analysis showing the relative protein level of Sirt6 after pcDNA3.1 Sirt6 and Sirt6 siRNA transfection of podocytes in different groups. (G–I) Membrane potential of mitochondrial in HPC cells were detected by JC-1 probe. (J) Morphology of mitochondria in HPC cells were detected by mito tracker red probe. *p < 0.05, **p < 0.01 vs NG.