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. 2024 Jul 31;46(2):2377785. doi: 10.1080/0886022X.2024.2377785

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 7. — Regulation of Sirt6 expression and analysis of Nrf2, GPX4, Nephrin in HPC cells. (A)The protein levels of Sirt6 in different transfection conditions detected by Western blot. 1.NG; 2. Lipofectamin 3000 2.5 μl + pcDNA3.1 Sirt6 2 μg + P3000 4 μl; 3. Lipofectamin 3000 3.5 μl + pcDNA3.1 Sirt6 2 μg + P3000 4 μl; 4. Lipofectamin 3000 3.5 μl + pcDNA3.1 Sirt6 4 μg + P3000 4 μl. *p < 0.05, **p < 0.01 vs NG. (B–I) The expression of Nrf2, GPX4 and Nephrin in HPC cells detected by Western blot and PCR. (J) Double immunofluorescence staining of Sirt6 (green) and GPX4 (red) in HPC cells (×400). NG: normal glucose; HG: high glucose; HG + Sirt6 + ML385: HG + pcDNA3.1 Sirt6 + ML385 (5 μM); HG + Sirt6: HG + pcDNA3.1 Sirt6. *p < 0.05, **p < 0.01.