Table 1.
Purification of L-fucose dehydrogenase from rabbit liver
| Fraction | Volume |
Total protein |
Total activity |
Specific activity |
Purification |
Yield |
|---|---|---|---|---|---|---|
| mL | Mg | μmol.min-1 | μmol.min-1.mg-1 | X-fold | % | |
| Homogenate (Supernatant)a | 195.0 | ND | ND | ND | ND | ND |
| PEG 4000 (30–50%) | 180.0 | 6244.7 | 17.17 | 0.003 | 1 | 100.0 |
| DEAE Sepharose | 28.0 | 831.3 | 7.72 | 0.009 | 3 | 40.5 |
| pH = 5.0 (Supernatant) | 10.0 | 113.0 | 2.33 | 0.021 | 8 | 13.6 |
| 3M NaCl | 2.5 | 26.0 | 1.39 | 0.053 | 19 | 8.1 |
| Superdex 200 16/60 HiLoad | 9.0 | 6.8 | 1.00 | 0.147 | 54 | 5.8 |
| Reactive Red 120b | 2.0 | 0.1 | 0.09 | 0.926 | 337 | 0.5 |
ND, not determined.
a
The enzyme activity was undetectable in the homogenate and was measurable only after homogenate fractionation with PEG 4000.
b
The data represent values only for the most purified fraction.