| Composition | Volume (10ml) | Final concentration |
|---|---|---|
| Peri-implantation culture medium base | 7ml | 70% (vol/vol) |
| Inactivated FBS | 3ml | 30% (vol/vol) |
| β-estradiol (10µM stock) | 8µl | 8nM |
| Progesterone (1mg/ml stock) | 2µl | 200ng/ml |
| NAC (50mM stock) | 5µl | 25µM |
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