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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Nat Protoc. 2023 Oct 11;18(12):3662–3689. doi: 10.1038/s41596-023-00891-y
Composition Volume (1ml) Final concentration
DMEM (low glucose, pyruvate, no glutamine, no phenol red) 969µl
GlutaMAX 10µl 1% (vol/vol)
Pen/strep 10µl 1% (vol/vol)
HEPES 11µl 11mM