| Composition | Volume (1ml) | Final concentration |
|---|---|---|
| DMEM (low glucose, pyruvate, no glutamine, no phenol red) | 969µl | |
| GlutaMAX | 10µl | 1% (vol/vol) |
| Pen/strep | 10µl | 1% (vol/vol) |
| HEPES | 11µl | 11mM |
| Composition | Volume (1ml) | Final concentration |
|---|---|---|
| DMEM (low glucose, pyruvate, no glutamine, no phenol red) | 969µl | |
| GlutaMAX | 10µl | 1% (vol/vol) |
| Pen/strep | 10µl | 1% (vol/vol) |
| HEPES | 11µl | 11mM |